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Protective Effect of Eucalyptus Oil Against Pulmonary Destruction and Inflammation in COPD Rats

Received: 23 January 2017     Accepted: 13 February 2017     Published: 1 March 2017
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Abstract

Eucalyptus oil (EO), an essential oil isolated from Eucalyptus leaves, was examined for its effect on LPS and Klebsiella pneumoniae - induced COPD in rats. The COPD model was induced by instilling intratracheally with LPS and Klebsiella pneumoniae (K. P). The test compound, EO (30, 100 and 300 mg/kg), Prednisone Acetate (10 mg/kg) or vehicle was instilled intragastrically after three weeks exposure of LPS and K. P, lasted for 4 weeks. EO significantly reduced amounts of inflammatory cells in bronchoalveolar lavage fluid (BALF) and blood, and decreased bronchiolitis, emphysematous changes and thickness of bronchioles. It also significantly reduced the increased AB-PAS-positive goblet cells in bronchioles. Prednisone Acetate attenuated pulmonary inflammation and airway mucus hypersecretion, but no significant difference was found on emphysema. Pretreatment with EO markly reduced the production of proinflammatory cytokines TNF-α and IL-β in lung homogenate, significantly decreased the elevated malondialdehyde (MDA) level and and increased superoxide dismutase (SOD) activity. These findings indicate that EO could exert an protective effect against LPS plus K. P-induced lung indury via inhibition of proinflammatory cytokines production and improvement of anti-oxidant status. Our results provide evidence that EO might have its potential to be a proper candidate drug in the treatment of COPD.

Published in Journal of Diseases and Medicinal Plants (Volume 3, Issue 1)
DOI 10.11648/j.jdmp.20170301.14
Page(s) 17-22
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Eucalyptus Globulus, Lipopolysaccharide, Cytokine, Chronic Obstructive Pulmonary Disease

References
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Cite This Article
  • APA Style

    Lin Wang, Jianbo Sun, Wanzhong Li, Yanna Lv, Weiwei Shi, et al. (2017). Protective Effect of Eucalyptus Oil Against Pulmonary Destruction and Inflammation in COPD Rats. Journal of Diseases and Medicinal Plants, 3(1), 17-22. https://doi.org/10.11648/j.jdmp.20170301.14

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    ACS Style

    Lin Wang; Jianbo Sun; Wanzhong Li; Yanna Lv; Weiwei Shi, et al. Protective Effect of Eucalyptus Oil Against Pulmonary Destruction and Inflammation in COPD Rats. J. Dis. Med. Plants 2017, 3(1), 17-22. doi: 10.11648/j.jdmp.20170301.14

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    AMA Style

    Lin Wang, Jianbo Sun, Wanzhong Li, Yanna Lv, Weiwei Shi, et al. Protective Effect of Eucalyptus Oil Against Pulmonary Destruction and Inflammation in COPD Rats. J Dis Med Plants. 2017;3(1):17-22. doi: 10.11648/j.jdmp.20170301.14

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  • @article{10.11648/j.jdmp.20170301.14,
      author = {Lin Wang and Jianbo Sun and Wanzhong Li and Yanna Lv and Weiwei Shi and Chunzhen Zhao},
      title = {Protective Effect of Eucalyptus Oil Against Pulmonary Destruction and Inflammation in COPD Rats},
      journal = {Journal of Diseases and Medicinal Plants},
      volume = {3},
      number = {1},
      pages = {17-22},
      doi = {10.11648/j.jdmp.20170301.14},
      url = {https://doi.org/10.11648/j.jdmp.20170301.14},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jdmp.20170301.14},
      abstract = {Eucalyptus oil (EO), an essential oil isolated from Eucalyptus leaves, was examined for its effect on LPS and Klebsiella pneumoniae - induced COPD in rats. The COPD model was induced by instilling intratracheally with LPS and Klebsiella pneumoniae (K. P). The test compound, EO (30, 100 and 300 mg/kg), Prednisone Acetate (10 mg/kg) or vehicle was instilled intragastrically after three weeks exposure of LPS and K. P, lasted for 4 weeks. EO significantly reduced amounts of inflammatory cells in bronchoalveolar lavage fluid (BALF) and blood, and decreased bronchiolitis, emphysematous changes and thickness of bronchioles. It also significantly reduced the increased AB-PAS-positive goblet cells in bronchioles. Prednisone Acetate attenuated pulmonary inflammation and airway mucus hypersecretion, but no significant difference was found on emphysema. Pretreatment with EO markly reduced the production of proinflammatory cytokines TNF-α and IL-β in lung homogenate, significantly decreased the elevated malondialdehyde (MDA) level and and increased superoxide dismutase (SOD) activity. These findings indicate that EO could exert an protective effect against LPS plus K. P-induced lung indury via inhibition of proinflammatory cytokines production and improvement of anti-oxidant status. Our results provide evidence that EO might have its potential to be a proper candidate drug in the treatment of COPD.},
     year = {2017}
    }
    

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  • TY  - JOUR
    T1  - Protective Effect of Eucalyptus Oil Against Pulmonary Destruction and Inflammation in COPD Rats
    AU  - Lin Wang
    AU  - Jianbo Sun
    AU  - Wanzhong Li
    AU  - Yanna Lv
    AU  - Weiwei Shi
    AU  - Chunzhen Zhao
    Y1  - 2017/03/01
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    N1  - https://doi.org/10.11648/j.jdmp.20170301.14
    DO  - 10.11648/j.jdmp.20170301.14
    T2  - Journal of Diseases and Medicinal Plants
    JF  - Journal of Diseases and Medicinal Plants
    JO  - Journal of Diseases and Medicinal Plants
    SP  - 17
    EP  - 22
    PB  - Science Publishing Group
    SN  - 2469-8210
    UR  - https://doi.org/10.11648/j.jdmp.20170301.14
    AB  - Eucalyptus oil (EO), an essential oil isolated from Eucalyptus leaves, was examined for its effect on LPS and Klebsiella pneumoniae - induced COPD in rats. The COPD model was induced by instilling intratracheally with LPS and Klebsiella pneumoniae (K. P). The test compound, EO (30, 100 and 300 mg/kg), Prednisone Acetate (10 mg/kg) or vehicle was instilled intragastrically after three weeks exposure of LPS and K. P, lasted for 4 weeks. EO significantly reduced amounts of inflammatory cells in bronchoalveolar lavage fluid (BALF) and blood, and decreased bronchiolitis, emphysematous changes and thickness of bronchioles. It also significantly reduced the increased AB-PAS-positive goblet cells in bronchioles. Prednisone Acetate attenuated pulmonary inflammation and airway mucus hypersecretion, but no significant difference was found on emphysema. Pretreatment with EO markly reduced the production of proinflammatory cytokines TNF-α and IL-β in lung homogenate, significantly decreased the elevated malondialdehyde (MDA) level and and increased superoxide dismutase (SOD) activity. These findings indicate that EO could exert an protective effect against LPS plus K. P-induced lung indury via inhibition of proinflammatory cytokines production and improvement of anti-oxidant status. Our results provide evidence that EO might have its potential to be a proper candidate drug in the treatment of COPD.
    VL  - 3
    IS  - 1
    ER  - 

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Author Information
  • Department of Pharmacology and Applied Pharmacology Laboratory, Laboratory for Cognitive Neuroscience, Weifang Medical University, Weifang, China

  • Department of Neurosurgery, Shouguang People’s Hospital, Shouguang, China

  • Department of Pharmacology and Applied Pharmacology Laboratory, Laboratory for Cognitive Neuroscience, Weifang Medical University, Weifang, China

  • Department of Pharmacology and Applied Pharmacology Laboratory, Laboratory for Cognitive Neuroscience, Weifang Medical University, Weifang, China

  • Department of Pharmacology and Applied Pharmacology Laboratory, Laboratory for Cognitive Neuroscience, Weifang Medical University, Weifang, China

  • Department of Pharmacology and Applied Pharmacology Laboratory, Laboratory for Cognitive Neuroscience, Weifang Medical University, Weifang, China

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